Anti-muchinjika-kusvibiswa, yakavimbika kuongororwa kweDNA inogona kuratidza chokwadi mumakona akavanzika

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Mumabhaisikopo mazhinji nemabhuku ezvinyorwa, marabhoritari ezvekuferefeta anoonekwa sehupenyu hwakakosha uye hwakakosha, kunyanya iyo DNA yekuzivikanwa bvunzo chiitiko inowanzova kiyi yekuwana zviratidzo uye kugadzirisa nyaya.Nekudaro, kana chokwadi chemhedzisiro yebvunzo yaunzwa isina chokwadi, hazvizove humbowo hwepamutemo, ndoda kuburitsa chokwadi mumakona akavanzika.Pane chinhu chakakosha chemuviri nekemikari icho marabhoritari ezvekuongororwa anofanira kubata nehungwaru, uye kudzivirira sampuli dzeDNA kuti dziongororwe kuti dzisasvibiswa murabhoritari.Parizvino, pane zvikonzero zvakasiyana zvekusvibiswa kweDNA.Pakati pavo, mukana wekuchinjika-kusvibiswa ndiyo yakakura.
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Kufanana nemamwe marabhoritari, zvinhu zvinodyiwa uye zvishandiso mumarabhoritari epamberi zvakasvibiswa muzviitiko zvakawanda.Kunyanya, kutapukira-kutapukira pakati pemasampuli eDNA kunoenderana nePCR maitiro, zvimwe zvinhu zvekuyedza uye humbowo hwemuviri, uye vanoedza ivo pachavo ndivo vanonyanya kunetsa kuona.Aya masara ehutsvina anosanganisira masero ebhayoloji, ropa, matishu, pamwe nekuyedza reagents, madhishi ekugezesa uye kumwe kusvibiswa.

Zvakakodzera kusimbisa kuti reusable glassware mu forensic marabhoritari akadai sampuli midziyo, reagent mabhodhoro, test tubes, pipettes, flasks, petri ndiro, etc. zvikanganiso zvinotungamirira kukuzivikanwa kusina kunaka uye kuongorora mhedziso.

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Iyi mhando yekusvibiswa kwegirazi ndeyekutyisidzira kuri pachena kune mhinduro dzebvunzo, saka chii chakakosha kuigadzirisa?

Chekutanga pane zvese, kana inofungidzirwa kuti DNA-yakasvibiswa mamiriro yawanikwa, mibairo yebvunzo inofanirwa kutariswazve nenguva kuti idzore chikanganiso.Ichi ndicho chekutanga chekutanga.

Wobva waita zviteshi zvekudzora zvemhando pazvinhu zvekuedza zvinodyiwa, zvinosanganisira midziyo yegirazi, reagents, nezvimwe, kuti uwedzere kusimbisa kwakabva kusvibiswa.

Pahwaro uhwu, vandudza nzira dzekuchenesa dzeglassware kugadzirisa zvikanganiso, kuitira kudzivirira zvikanganiso zvakafanana kuti zvisaitike zvakare.

Chetatu, chete nekusimbisa iyo yese inopokana nekusvibisa uye decontamination matanho erabhoritari kuti igadzire manejimendi ane masangano anogona kuita optimization yemaitiro ekuchenesa kuve nechinangwa uye kunoshanda.

Muchokwadi, rabhoritari yezvemberi inofanirwa kuve nenzvimbo yakatsaurirwa uye yakazvimirira yekuongororwa kweDNA kuona kuti kusvibiswa kwakadzikiswa mumhando dzakasiyana dzekuedza.Semuenzaniso, kugamuchirwa kwemhosva uye nzvimbo yekuchengetera sampuli, sampuli yeDNA extraction area, DNA amplification area, DNA yekuona nzvimbo, nzvimbo yekuongorora yekutanga, nzvimbo yekuongorora mhedzisiro, nzvimbo yekugadzirira, DNA amplification nzvimbo, yekuona buffer nzvimbo, nezvimwe zvakadaro.Pakati pavo, kuchenesa glassware munzvimbo yekugadzirira kuchabatsira zvikuru kuwedzera mukana wekukundikana kwemigumisiro yekuedza.

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Izvo zvinofanirwa kucherechedzwa kuti akawanda enyanzvi marabhoritari, kusanganisira forensic marabhoritari, achiri kushandisa isingashande yemanyorero nzira dzekuchenesa kugadzirisa dambudziko rekusvibisa masara mune glassware.Asi nzira iyi hainyatso kuvandudza njodzi yemunhu yekutapukirwa.

Zvakare, izvo zvisingabatsiri zvekuchenesa manyore egirazi zvinoenda kure kupfuura izvi.

Mkuchenesa magirazi egirazi hakuzongotadza kunyatso kuchenesa rabhoritari yegirazi uye kukanganisa magumo ekupedzisira ekuongororwa kweDNA nekuzivikanwa, zvinounzawo nhevedzano yekupokana sekutambisa zviwanikwa, kuoma kwekuchenesa mashandiro, uye njodzi dzekuchengetedza vashandi vemurabhoritari. .Panguva ino, kushandiswa kwemishonga otomatiki glassware washerrinoshandiswa mumarabhoritari epasi rose rinobatsira kugadzirisa mamiriro ezvinhu aya.

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Laboratory glassware washerinogona kunyatso kuchenesa zvakasiyana-siyana zverabhoritari glassware nenzira yakachengeteka, batch, uye nehungwaru kuita kuti ienderane nemirairo yakakodzera yeGMP neFDA.Kuenzaniswa neiyo manual yekuchenesa nzira, iyo Washer yeLabhoritariinogona kuteedzera maitiro ekuchenesa mukati memaitiro ese, ayo anobatsira kuwana akakosha data rekodhi mune akasiyana siyana.Aya data ane kukosha kukuru pakubviswa kwezvakasara kusanganisira kusvibiswa kwakatarisana nekuongororwa kweDNA.Kunyanya kana pane misiyano uye kusava nechokwadi pamusoro pemigumisiro yekuedza!

Nekuenderera mberi kwekuvandudza matekinoroji akakodzera, forensic marabhoritari anozotora mamwe mabasa mukugadzirisa nyaya.Nenzira iyi, izvo zvinodikanwa kune chero marabhoritari bvunzo mhinduro uye kumira uye kurongeka zvinongoramba zvichiwedzera.Nzira dzinosanganisira kuongororwa kweDNA dzinogona chete kuvimbisa mibairo yakachena uye kuwana mhedziso chaiyo uye humbowo kana vakabudirira mukubvisa tsvina.Ichi chinhu chinofanira kurangarirwa yega yega forensic laboratory.


Nguva yekutumira: Kubvumbi-02-2021